Fig. 3. A. Effect of energy depletion on the percentage of annexin V binding erythrocytes. Original histogram and arithmetic means ± SEM (n = 10) of annexin V binding of erythrocytes following exposure for 48 h to glucose containing glucose Ringer solution (black line and white bar) and Ringer solution without glucose (red line and black bar). ****(p<0.001) indicates significant difference from the presence of glucose (ANOVA). B. Effect of energy depletion on erythrocyte forward scatter. Original histogram and arithmetic means ± SEM (n = 10) of forward scatter of erythrocytes following exposure for 48 h to glucose containing Ringer solution (black line and white bar) and Ringer solution without glucose (red line and black bar). ***(p<0.005) indicates significant difference from the presence of glucose (ANOVA). C. Effect of energy depletion on cytosolic Ca²⁺ concentration. Original histogram and arithmetic means ± SEM (n = 10) of Fluo3 fluorescence reflecting cytosolic Ca²⁺ concentration in erythrocytes following exposure for 48 h to glucose-containing glucose Ringer solution (black line and white bar) or Ringer solution without glucose (red line and black bar). ****(p<0.001) indicates significant difference from the absence of glucose (ANOVA).